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tisdag 18 oktober 2016

Onko miesten ja naisten K1-vitamiinitarve sama painokiloa kohden?

 Otsikon kysymys: Ainakin naisilla K-vitamiinintarve riippuu fysiologisista vaiheista, kuten raskausajasta, imetyksestä, jolloin tarve on suurempi tai ainakin  "otettava huomioon", Lapsilla tarve on otettava huomuoon  varhaisvaiheessa synnytyksen jälkeisinä kuukausina.  Seuraava vaihe, jossa asiaa pohditaan on  Marevanin tarve loppuiällä eri tautien takia.


Biochem Biophys Res Commun. 2015 May 1;460(2):238-44. doi: 10.1016/j.bbrc.2015.03.018. Epub 2015 Mar 12.

YY1 positively regulates human UBIAD1 expression.


Vitamin K is involved in bone formation and blood coagulation. Natural vitamin K compounds are composed of the plant form phylloquinone (vitamin K1) and a series of bacterial menaquionones (MK-n; vitamin K2). Menadione (vitamin K3) is an artificial vitamin K compound.

MK-4 contains 4-isoprenyl as a side group in the 2-methyl-1,4-naphthoquinone common structure and has various bioactivities.

UbiA prenyltransferase domain containing 1 (UBIAD1 or TERE1) is the menaquinone-4 biosynthetic enzyme.

 UBIAD1 transcript expression significantly decreases in patients with prostate carcinoma and overexpressing  UBIAD1 inhibits proliferation of a tumour cell line.
 UBIAD1 mRNA expression is ubiquitous in mouse tissues, and higher UBIAD1 mRNA expression levels are detected in the brain, heart, kidneys and pancreas.

Several functions of UBIAD1 have been reported; however, regulation of the human UBIAD1 gene has not been elucidated.

Here we report cloning and characterisation of the human UBIAD1 promoter. A 5' rapid amplification of cDNA ends analysis revealed that the main transcriptional start site was 306 nucleotides upstream of the translation initiation codon.

 Deletion and mutation analyses revealed the functional importance of the YY1 consensus motif. Electrophoretic gel mobility shift and chromatin immunoprecipitation assays demonstrated that YY1 binds the UBIAD1 promoter in vitro and in vivo.

In addition, YY1 small interfering RNA decreased endogenous UBIAD1 mRNA expression and UBIAD1 conversion activity. These results suggest that YY1 up-regulates UBIAD1 expression and UBIAD1 conversion activity through the UBIAD1 promoter.


Gene regulation; UBIAD1; Vitamin K; YY1

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