Neurobiol Dis. 2015 Oct;82:504-515. doi: 10.1016/j.nbd.2015.09.007. Epub 2015 Sep 24.
A nutrient combination designed to enhance synapse formation and function improves outcome in experimental spinal cord injury.
Pallier PN1, Poddighe L2, Zbarsky V1, Kostusiak M1, Choudhury R1, Hart T1, Burguillos MA1, Musbahi O1, Groenendijk M3, Sijben JW3, deWilde MC3, Quartu M2, Priestley JV1, Michael-Titus AT4.
Abstract
Spinal
cord injury leads to major neurological impairment for which there is
currently no effective treatment. Recent clinical trials have
demonstrated the efficacy of Fortasyn® Connect in Alzheimer's disease.
Fortasyn® Connect is a specific multi-nutrient combination
containing DHA, EPA,
choline,
uridine monophosphate (UMP),
phospholipids,
and various vitamins.
We examined the effect of Fortasyn® Connect in a rat compression model of spinal cord injury.
For 4 or 9 weeks following the injury, rats were fed either a control diet or a diet enriched with low, medium, or high doses of Fortasyn® Connect. The medium-dose Fortasyn® Connect-enriched diet showed significant efficacy in locomotor recovery after 9 weeks of supplementation, along with protection of spinal cord tissue (increased neuronal and oligodendrocyte survival, decreased microglial activation, and preserved axonal integrity). Rats fed the high-dose Fortasyn® Connect-enriched diet for 4 weeks showed a much greater enhancement of locomotor recovery, with a faster onset, than rats fed the medium dose. Bladder function recovered quicker in these rats than in rats fed the control diet. Their spinal cord tissues showed a smaller lesion, reduced neuronal and oligodendrocyte loss, decreased neuroinflammatory response, reduced astrocytosis and levels of inhibitory chondroitin sulphate proteoglycans, and better preservation of serotonergic axons than those of rats fed the control diet. These results suggest that this multi-nutrient preparation has a marked therapeutic potential in spinal cord injury, and raise the possibility that this original approach could be used to support spinal cord injured patients.
containing DHA, EPA,
choline,
uridine monophosphate (UMP),
phospholipids,
and various vitamins.
We examined the effect of Fortasyn® Connect in a rat compression model of spinal cord injury.
For 4 or 9 weeks following the injury, rats were fed either a control diet or a diet enriched with low, medium, or high doses of Fortasyn® Connect. The medium-dose Fortasyn® Connect-enriched diet showed significant efficacy in locomotor recovery after 9 weeks of supplementation, along with protection of spinal cord tissue (increased neuronal and oligodendrocyte survival, decreased microglial activation, and preserved axonal integrity). Rats fed the high-dose Fortasyn® Connect-enriched diet for 4 weeks showed a much greater enhancement of locomotor recovery, with a faster onset, than rats fed the medium dose. Bladder function recovered quicker in these rats than in rats fed the control diet. Their spinal cord tissues showed a smaller lesion, reduced neuronal and oligodendrocyte loss, decreased neuroinflammatory response, reduced astrocytosis and levels of inhibitory chondroitin sulphate proteoglycans, and better preservation of serotonergic axons than those of rats fed the control diet. These results suggest that this multi-nutrient preparation has a marked therapeutic potential in spinal cord injury, and raise the possibility that this original approach could be used to support spinal cord injured patients.
Crown Copyright © 2015. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Dietary supplementation; Fortasyn connect; Neuroplasticity; Neuroprotection; Spinal cord injury
2)
3)
Dietary supplementation; Fortasyn connect; Neuroplasticity; Neuroprotection; Spinal cord injury
2)
Neurobiol Aging. 2015 Jan;36(1):344-51. doi: 10.1016/j.neurobiolaging.2014.07.021. Epub 2014 Jul 24.
Specific multi-nutrient enriched diet enhances hippocampal cholinergic transmission in aged rats.
Abstract
Fortasyn Connect
(FC) is a specific nutrient combination designed to target synaptic
dysfunction in Alzheimer's disease by providing neuronal membrane
precursors and other supportive nutrients. The aim of the present study
was to investigate the effects of FC on hippocampal cholinergic
neurotransmission in association with its effects on synaptic membrane
formation in aged rats. Eighteen-month-old male Wistar rats were
randomized to receive a control diet for 4 weeks or an FC-enriched diet
for 4 or 6 weeks. At the end of the dietary treatments, acetylcholine
(ACh) release was investigated by in vivo microdialysis in the right
hippocampi. On completion of microdialysis studies, the rats were
sacrificed, and the left hippocampi were obtained to determine the
levels of choline, ACh, membrane phospholipids, synaptic proteins, and
choline acetyltransferase. Our results revealed that supplementation
with FC diet for 4 or 6 weeks, significantly enhanced basal and
stimulated hippocampal ACh release and ACh tissue levels, along with
levels of phospholipids. Feeding rats the FC diet for 6 weeks
significantly increased the levels of choline acetyltransferase, the
presynaptic marker Synapsin-1, and the postsynaptic marker PSD-95, but
decreased levels of Nogo-A, a neurite outgrowth inhibitor. These data
show that the FC diet enhances hippocampal cholinergic neurotransmission
in aged rats and suggest that this effect is mediated by enhanced
synaptic membrane formation. These data provide further insight into
cellular and molecular mechanisms by which FC may support memory
processes in Alzheimer's disease.
Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Acetylcholine release; Aged rat; Alzheimer's disease; Choline acetyltransferase; Cholinergic neurotransmission; Fortasyn connect; Phospholipid; Souvenaid; Synaptic membrane3)
Front Neurosci. 2017 Aug 4;11:440. doi: 10.3389/fnins.2017.00440. eCollection 2017.
High Content Analysis of Hippocampal Neuron-Astrocyte Co-cultures Shows a Positive Effect of Fortasyn Connect on Neuronal Survival and Postsynaptic Maturation.
Abstract
Neuronal
and synaptic membranes are composed of a phospholipid bilayer.
Supplementation with dietary precursors for phospholipid synthesis
-docosahexaenoic acid (DHA), uridine and choline- has been shown to increase neurite outgrowth and synaptogenesis both in vivo and in vitro.
A role for multi-nutrient intervention with specific precursors and
cofactors has recently emerged in early Alzheimer's disease, which is
characterized by decreased synapse numbers in the hippocampus. Moreover,
the medical food Souvenaid, containing the specific nutrient
combination Fortasyn
Connect (FC), improves memory performance in early Alzheimer's disease
patients, possibly via maintaining brain connectivity. This suggests an
effect of FC on synapses, but the underlying cellular mechanism is not
fully understood.
Therefore, we investigated the effect of FC (consisting of DHA, eicosapentaenoic acid (EPA), uridine, choline, phospholipids, folic acid, vitamins B12, B6, C and E, and selenium), on synaptogenesis by supplementing it to primary neuron-astrocyte co-cultures, a cellular model that mimics metabolic dependencies in the brain. We measured neuronal developmental processes using high content screening in an automated manner, including neuronal survival, neurite morphology, as well as the formation and maturation of synapses. Here, we show that FC supplementation resulted in increased numbers of neurons without affecting astrocyte number. Furthermore, FC increased postsynaptic PSD95 levels in both immature and mature synapses. These findings suggest that supplementation with FC to neuron-astrocyte co-cultures increased both neuronal survival and the maturation of postsynaptic terminals, which might aid the functional interpretation of FC-based intervention strategies in neurological diseases characterized by neuronal loss and impaired synaptic functioning.
Therefore, we investigated the effect of FC (consisting of DHA, eicosapentaenoic acid (EPA), uridine, choline, phospholipids, folic acid, vitamins B12, B6, C and E, and selenium), on synaptogenesis by supplementing it to primary neuron-astrocyte co-cultures, a cellular model that mimics metabolic dependencies in the brain. We measured neuronal developmental processes using high content screening in an automated manner, including neuronal survival, neurite morphology, as well as the formation and maturation of synapses. Here, we show that FC supplementation resulted in increased numbers of neurons without affecting astrocyte number. Furthermore, FC increased postsynaptic PSD95 levels in both immature and mature synapses. These findings suggest that supplementation with FC to neuron-astrocyte co-cultures increased both neuronal survival and the maturation of postsynaptic terminals, which might aid the functional interpretation of FC-based intervention strategies in neurological diseases characterized by neuronal loss and impaired synaptic functioning.
KEYWORDS:
method; neuron-astrocyte co-culture; nutritional intervention; phospholipids; synapse formation- PMID:
- 28824363
- PMCID:
- PMC5543085
- DOI:
- 10.3389/fnins.2017.00440
- Kommentti: kannatan tätä kombinaatiota. Aivan välttämättömiä ovat foolihappo, B12 ja ehkä B6 ( B6 tehostamassa että ei tapahdu malnutritiota sen takia että typen fiksausta aminohapoiksi tapahtuu liian heikosti , B6 myös tehosta seriinin tallettamista aktiivina mm myeliinin synteesiin ja poimii seriiniä täten fosfolipidien metaboliaan salvage reiteillekin)
- UMP on sikäli eduksi, että sen muodostuksen heikkous on tyypillistä sokeriaineenvaihdunnan vajeissa.
- UDP, UTP - energia kuuluu hiilihydraattiaineenvaihduntaan ja samalla myös rikkipitoisten kalvorakenteiden kehittymsieen (kehon oma hepariini jokossa ja muut negatiiviseen koagulaatiotasaoainoon kuulu viin tekijöihin- sulfaattiaineenvaihduntaan saa näistä kombinaatioista oikaisua sokeriaineenvaihdunnan avunstamisen kautta.
- Tämä kohta on ollut sellainen musta-aukko, johon en kuvitellut löytyvänkään mitään ainetta antaa. avuksi. Olen ajatellut että ainoa mahdollisuus oikaista U- aineenvaihdunnan bermudaa on vain normaalin verensokerin pitäminen ja liikunta. NNR 2012 suositusten lisäksi. ja varmsitaa metylaatiot B12, foolihappo, ettei tapahdu liiallista U putoamista genomista.
- Foolihappoa saisi antaa kyllä densiteetillä. biopteriinin toiminnan elvyttämsieksi.
- B12- antoon pitäisi varmaan liittää maksaravinto. uncertainti.
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